Use of Real-Time Quantitative PCR to Validate the Results of cDNA Array and Differential Display PCR Technologies
نویسندگان
چکیده
منابع مشابه
Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies.
Real-time reverse transcription polymerase chain reaction (RT-PCR) methods that monitor product accumulation were adapted for the validation of differentially expressed genes. We describe a real-time quantitative PCR assay that uses SYBR Green I dye-based detection and product melting curve analysis to validate differentially expressed genes identified by gene expression profiling technologies....
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We previously described a targeted genomic differential display method (TGDD: Broude NE, Chandra A, Smith CL. Differential display of genomic subsets containing specific interspersed repeats. Proc. Natl. Acad. Sci. USA 1997;94:4548-53). In that method, presently characterized as method I, targeting was accomplished by capturing DNA fragments containing specific a sequence by hybridization with ...
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ژورنال
عنوان ژورنال: Methods
سال: 2001
ISSN: 1046-2023
DOI: 10.1006/meth.2001.1266